Process for the preparation of platelet growth factors extract

Details Process for the preparation of platelet growth factors extract Process for the preparation of platelet growth factors extract

1996-03-20

1998-11-10

Tsang, Cecilia J.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

514 12, 514 21, 530380, 530422, A61K 3817, C07K 14435

Patent

active

058344187

ABSTRACT:
The present invention relates to a process for the preparation of porcine platelet-extract containing matured growth factors, which comprises the steps of: a) centrifuging whole porcine blood at about 1000 g to about 5000 g to isolate the platelets from the platelet-rich-plasma; b) resuspending the isolated platelets of step a) in Plasma-Lyte A and centrifuging to concentrate the platelets; c) washing the concentrated platelets of step b); d) lyophilizing the washed platelets of step c); whereby causing lysis of the platelets and producing the platelet extract in optimal amount.

REFERENCES:
Assoian RK et al., J. Biol. Chem., 1983, 258:7155-7160.
Carter et al., In Growth Factors and Other Aspects of Wound Healing: Biological and Clinical Implications, Alan R. Liss, Inc., 1988, 303-317.
Greenhalgh DG et al., Wound. Rep. Reg., 1993, 1:69-81.
Knighton et al., In Growth Factors and Other Aspects of Wound Healing: Biological and Clinical Implications, Alan R. Liss, Inc., 1988, 319-329.
Knighton et al., Ann. Surg., 1986, 204:322-330.
Lowry method, J. Biol. Chem., 1951, 193:265-275.
Lynch SE et al., J. Clin. Invest., 1989, 84:640-646.
Sara VR et al., Physiol. Rev., 1990, 70:591-614.
Stroobant P & Waterfield MD, EMBO J., 1984, 12:2963-2967.
Assoian et al. J. Biol. Chem., vol. 258:7155-60 (1983).
Carter et al., In Growth Factors and Other Aspects of Wound Healing, Alan R. Liss, Inc., 1988, pp. 303-317.

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