DNA encoding enzyme, recombinant DNA and enzyme, transformant, a

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435200, 435100, 435274, C12N 926, C12N 924, C12P 1912, C08B 3004

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active

058342877

ABSTRACT:
Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.

REFERENCES:
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patent: 5472863 (1995-12-01), Maruta et al.
patent: 5556781 (1996-09-01), Kubota et al.
E. M. Southern, Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis, J. Mol. Biol., vol. 98, pp. 503-517, 1975.
Enzyme Kinetics of Amylases and Related Enzymes, Handbook of Amylases and Related Enzymes, pp. 1-9, 1988.
U. K. Laemmli, Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature, vol. 227, pp. 680-685, 15 Aug. 1970.
J. Sambrook et al, Molecular Cloning a Laboratory Manual, Second Edition, published by Cold Spring Harbor Laboratory Press, 1989.
Maruta et al., Biochimica Et Biophysica Acta, "Cloning and Sequencing of Trehalose Biosynthesis Genes from Arthrobacter sp. Q36", No. 1289, 1996, pp. 10-13.

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