Process for deodorizing fermented culture broths with superheate

Details Process for deodorizing fermented culture broths with superheate Process for deodorizing fermented culture broths with superheate

1997-01-15

1998-11-10

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435 41, 435219, 435264, C12N 900, C12N 950, C12P 100, D06M 1600

Patent

active

058342753

DESCRIPTION:

BRIEF SUMMARY
BACKGROUND OF THE INVENTION

1. Field of the Invention
The biotechnological production of useful materials and mixtures thereof by cultivation of selected microorganism populations is now of considerable industrial significance. The production of washing- and/or cleaning-active enzymes, more particularly from the classes of proteases, cellulases, lipases and amylases, and the production of pharmacologically active useful materials are mentioned purely by way of example in this regard. Aqueous preparations of intracellular and/or extracellular useful materials contaminated by a number of impurities, for example parts of the nutrient medium, accumulate as primary process products. Extensive prior art literature is available on the working up of such culture broths, cf. for example the article by W. Rahse et al. entitled "Mikrofiltration von Fermenterbruhen (Microfiltration of Fermenter Broths)" in Chem.-Ing.-Techn. 57 (1985), No. 9,747 to 753 and the primary literature cited therein. Examples of relevant patents include, for example, EP-B1 0 200 032, WO 93/16173 and earlier German patent applications DE 43 10 506 and DE 43 22 229.
2. Discussion of Related Art
The multistage working-up of these biotechnologically obtained aqueous culture solutions seeks in particular to remove unwanted constituents by optionally multistage filtration and/or by washing, generally followed by solidification of the aqueous preparation using solid carriers. In the bacteriological production of washing- and cleaning-active enzymes, which is of particular commercial relevance, a particular problem lies in the following situation: through the metabolism in the fermentation process, the biomass-containing fermenter broths contain small quantities of low molecular weight compounds, more particularly corresponding nitrogen compounds, which are distinguished by a highly obtrusive unpleasant odor and which can promote the degradation of the biotechnological useful materials to form other foul-smelling compounds. A typical example of these phenomena can be found in the biotechnological production of proteases or protease solutions which are intended for use in detergents, more particularly as a mixture component in laundry detergents. In addition to the prior art literature already mentioned, reference is also made in this regard to International patent application WO 91/2792. A biomass-containing fermenter broth containing around 70,000 protease units per gram (PU/g) is obtained using selected microorganisms (Bacillus licheniformis--ATCC 53926) by a process similar to the process described in German patent DE 29 25 427. In addition to dissolved proteases, salts, proteins and metabolism products, the fermenter broth also contains undissolved constituents, such as bacillus cells, residues of nutrient medium and mucilaginous substances. During the working-up of the fermenter broths (WO 92/11347), the proteases are stabilized and relatively coarse particles and undissolved constituents are removed by decantation or microfiltration. The protease solution is concentrated by ultrafiltration and subsequent evaporation in vacuo. The protease solution obtained is mixed with solid carrier materials and granulation aids and made up into enzyme granules for use in detergents.
Despite the elaborate purification and working-up processes, the concentrated protease solution still contains small quantities of low molecular weight nitrogen compounds with an unpleasant odor which can additionally accelerate degradation of the protease in storage to form other foul-smelling sulfur compounds, for example mercaptans or thioethers.
The problem addressed by the teaching according to the present invention was to remove these low molecular weight odorous compounds in an additional but simple step to such an extent that products of substantially neutral odor would be obtained, in particular after the usual encapsulation of the useful materials or mixtures thereof, self-initiated decomposition processes being ruled out or at least largely suppressed at the same time

REFERENCES:
patent: 4751003 (1988-06-01), Raehse et al.
patent: 5352604 (1994-10-01), Wilson et al.
patent: 5637560 (1997-06-01), Raehge et al.
W. Raehse et al., "Mikrofiltration von Fermenterbruehen" (Microfiltration of Fermenter Broths), Chem. Ing. Techn. 57 (1985) No. 9, pp. 747-753, and the Primary Literature Cited Therein.

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