Methodology for determining oxygen in biological systems

Details Methodology for determining oxygen in biological systems Methodology for determining oxygen in biological systems

1997-04-28

1998-11-10

Smith, Ruth S.

Surgery

Diagnostic testing

Measuring or detecting nonradioactive constituent of body...

600420, 324316, A61B 5055

Patent

active

058336010

ABSTRACT:
The invention provides apparatus and methods for measuring oxygen tensions in biological systems utilizing physiologically acceptable paramagnetic material, such as India ink or carbon black, and electron paramagnetic resonance (EPR) oximetry. India ink is introduced to the biological system and exposed to a magnetic field and an electromagnetic field in the 1-2 GHz range. The EPR spectra is then measured at the biological system to determine oxygen concentration. The EPR spectra is determined by an EPR spectrometer that adjusts the resonator to a single resonator frequency to compensate for movements of the biological system, such as a human or animal. The biological system can also include other in vivo tissues, cells, and cell cultures to directly measure pO.sub.2 non-destructively.

REFERENCES:
patent: 4560663 (1985-12-01), Nicksic et al.
patent: 4593248 (1986-06-01), Hyde et al.
patent: 4714886 (1987-12-01), Halpern
patent: 4803624 (1989-02-01), Pilbrow et al.
patent: 5204628 (1993-04-01), Konishi et al.
patent: 5233303 (1993-08-01), Bales et al.
patent: 5494030 (1996-02-01), Swartz et al.
Arie H. Bartal et al., "Malignant Melanoma Arising At Tattoo Sites Used For Radiotherapy Field Marking", The British Journal Of Radiology, pp. 913-914, vol. 53, No. 633, (Sep. 1980).
M. Brian Fennerty et al., "Effectiveness of India Ink As A Long-Term Colonic Mucosal Marker", The American Journal Of Gastroenterology, pp. 79-81, vol. 87, No. 1, (Jan. 1992).
William J. Whalen et al., "Skeletal Muscle PO2: Effect Of Inhaled And Topically Applied O2 and CO2", American Journal Of Physiology, pp. 973-980.
Thomas E. J. Gayeski et al., "Intracellular PO2 In Long Axis Of Individual Fibers In Working Dog Gracilis Muscle", The American Journal Of Physiology, pp. H1179-H1186.
H. M. Swartz et al., "Measurements Of Pertinent Concentrations Of Oxygen In Vivo", Magnetic Resonance In Medicine, pp. 333-339, vol. 20, No. 2, (Aug. 1991).
K. J. Liu et al., "Lithium Phthalocyanine: A Probe For Electron Paramagnetic Resonance Oximetry In Viable Biological Systems", Proceedings Of The National Academy Of Sciences, pp. 5438-5442, vol. 90, No. 12, (Jun. 1993).
J. M. Vanderkooi et al., "Oxygen In Mammalian Tissue: Methods Of Measurement And Affinites Of Various Reactions", American Journal Of Physiology, pp. C1131-C1150, vol. 260, No. 6, (Jun. 1991).
James F. Glockner et al., "In Vivo EPR Oximetry Using Two Novel Probes: Fusinite And Lithium Phthalocyanine", Oxygen Transport To Tissue XIV, pp. 229-234, Plenum Press, New York, (1992).
Ernst Epstein, "Surgical Gem", The Jorunal Of Dermatologic Surgery And Oncology, pp. 273-274, vol. 15, No. 3, (Mar. 1989).
H. M. Swartz et al., "The Use Of EPR For The Measurement Of The Concentration Of Oxygen In Vivo In Tissues Under Physiologically Pertinent Conditions And Concentrations", The Journal Of Dermatologic Surgery And Oncology, pp. 221-228, vol. 15, No. 3, (Mar. 1989).
J. D. Chapman, "Measurement Of Tumor Hypoxia By Invasive And Non-Invasive Procedures: A Review Of Recent Clinical Studies", Radiotherapy And Oncology, pp. 13-19, Suppl. 20, (1991).
Crepeau et al., "Communications--Composite Pulses In Time-Domain ESR", J. of Magnetic Resonance, vol. 84, pp. 184-190, (Aug. 1989).
Glockner et al., "In Vivo Oximetry Using A Nitroxide-Liposome System", J. Of Magnetic Resonance, vol. 20, pp. 123-133, (Jul. 1991).
Duret et al., "Oxygen Concentration Measurements Using The ESR Line Modification Of PcLi Molecules", Sensors And Actuators B Chemical, vol. B6, pp. 266-269, (Jan. 1992).
Smirnov et al., "Simultaneous Multi-Site EPR Spectroscopy In Vivo", Magnetic Resonance In Medicine, vol. 30, pp. 213-220, (Aug. 1993).
Swartz et al., "India Ink" A Potential Clinically Applicable EPR Oximetry Probe, Magnetic Resonance In Medicine, vol. 31, pp. 229-232, (Feb 1994).

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