Method for preparing cellobiose

Details Method for preparing cellobiose Method for preparing cellobiose

1990-10-16

1991-12-31

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 42, 435100, C12P 1924, C12P 3900, C12P 1912

Patent

active

050772058

ABSTRACT:
A method for preparing cellobiose from sucrose in a high yield at low cost without difficulty by using three enzymes of sucrose phosphorylase, glucose isomerase and cellobiose phosphorylase in the presence of orthophosphate. The additional method of the invention comprises 4 steps: to treat sucrose with sucrose phosphorylase in the presence of orthophosphate to produce fructose and glucose-1-phosphate; to treat the fructose obtained in the preceding step with glucose isomerase to produce glucose; to treat the glucose obtained in the preceding step and the glucose-1-phosphate obtained in the first step with cellobiose phosphorylase to produce cellobiose and orthophosphate; and to recover at least a part of cellobiose from the reaction mixture in the preceding step and to recycle at least a part of the remaining reaction mixture containing orthophosphate to the first step.

REFERENCES:
patent: 4410627 (1983-10-01), Lloyd et al.
patent: 4683203 (1986-01-01), Yoshioka et al.
ABS APS J63146800, J63-49100, Katayama et al., pub. date ABS. 7-1988.

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