Method for producing enzymatically active eucaryotic sod in bact

Details Method for producing enzymatically active eucaryotic sod in bact Method for producing enzymatically active eucaryotic sod in bact

1984-08-27

1988-05-03

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

4351723, 435189, 935 9, 935 14, C12P 2102, C12N 1500, C12N 902

Patent

active

047420049

ABSTRACT:
A method of producing enzymatically active eucaryotic superoxide dismutase or an analog thereof in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase or analog thereof comprising maintaining the bacterial cell under suitable conditions and in a suitable production medium. The production medium is supplemented with an amount of Cu++ so that the concentration of Cu++ in the medium is greater than about about 2 ppm.
The invention also concerns methods of recovering purified enzymatically active eucaryotic superoxide dismutase or analogs from bacterial cells producing the superoxide dismutase or analog.

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