Mycoplasma diagnostic assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 72, 435 792, 435870, 435973, 4352531, 436501, 5303895, G01N 3353

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active

055321348

ABSTRACT:
The present invention relates to a diagnostic assay utilizing the lipid associated membrane proteins of mycoplasmas to detect the presence of species-specific mycoplasma antibodies. The sensitive assay is useful for the detection and differentiation of mycoplasma antibodies specific for individual species in biological samples from humans and animals, and exhibits virtually no cross-reactivity between different mycoplasma species.

REFERENCES:
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Chapter 30 "Serodiagnosis", pp. 505-512 in Mycoplasmas Molecular Biology and Pathogenesis, Maniloff et al, editors, (American Society for Microbiology, Washington, D.C., 1992).
Bricker et al, "Association of Lipids with Integral Membrane Surface Proteins of Mycoplasma hyorhinis", Infect. Immun. 56(2):295-301 (Feb. 1988).
Morrison-Plummer et al, "An ELISA to Detect Monoclonal Antibodies Specific for Lipid Determinants of Mycoplasma pneumoniae", J. Immunol. Methods, 64:165-178, (1983).
Lee et al, "Comparative Studies of Three Serologic Methods for the Measurement of Mycoplasma pneumoniae Antibodies", Am. J. Clin. Pathol., 92(3):342-347, (Sep. 1989).
Cimolai et al "Immunological Cross-Reactivity of a Mycoplasma pneumoniae Membrane-Associated Protein Antigen with Mycoplasma genitalium and Acholeplasma laidlawii", J. Clin. Microbiol., 25(11):2136-2139 (Nov. 1987).
Lo et al (1989)--Abstract Only--"Identification of Mycoplasma incognitus . . . " Am J Trop Med Hyg 41:601-616.
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Stevens et al (1991) "Localization of the M. pneumoniae . . . " J. Bact 173:1041-1050.
Wang et al (1992) "High frequency Antibodies . . . " The Lancet 340:1312-1316.
Wise et al (1987)--Abstract Only--"Identification of Intrinsic and Extrinsic . . . ", Isr J Med Sci 23:469-473.
Riethman et al (1987) "Triton X-114 Phase Fractionation . . . " Infect. and Immun 55:1094-1100.
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Boyer et al (1989) "Lipid-Modified Suface Protein . . . " Infect. and Immun. 57:245-254.

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