Method for making stable, extracellular tyrosinase and synthesis

Details Method for making stable, extracellular tyrosinase and synthesis Method for making stable, extracellular tyrosinase and synthesis

1993-12-14

1996-01-23

Wax, Robert A.

Drug, bio-affecting and body treating compositions

Topical sun or radiation screening, or tanning preparations

435 691, 435212, 4352523, 43525235, 4353201, 424 62, 424574, A61K 742, C12P 2106, C12N 120, C12N 1500

Patent

active

054863511

ABSTRACT:
A process for the in vitro production of chemically modified polyphenolic polymer (PPP). First, stable, highly active extracellular tyrosinase is produced from genetically transformed microorganism such as Streptomyces antibioticus. The tyrosinase is then incubated with a reaction substrate such as 1-tyrosine, hydrolyzed protein, or an oligopeptide in combination with 1-tyrosine. The ratio of the oligopeptide/tyrosine combination as well as variation in the concentration of tyrosinase can be used to modify the color, the molecular size, and the spectral absorbance properties of the PPP produced. Alternatively, or additionally, oxidants such as hydrogen peroxide or hypochlorite can be used to modify the color of the PPP, regardless of the method used to produce the PPP, and the PPP can subsequently be fractionated using molecular weight cut-off ultrafiltration. Organic solvents can also be used in the method of making PPP to produce PPPs having variable but reproducible physical properties.

REFERENCES:
patent: 5188844 (1993-02-01), Ahene et al.
Katz et al. "Cloning and expression of the tyrosinase gene . . . " Journ. Gen. Micro. (1983) 129, pp. 2703-2714.
Doddema "Site specific hydrocylation . . . " Biotechnology and Bioengineering (1988) 32, pp. 716-718.
Yoshimoto et al. "Extracellular tyrosinase . . . " J. Biochem. (1985) 97, pp. 1747-1754.
Prota "Progress in the Chemistry of Melanins . . . " Med. Res. Reviews (1988) vol. 8, No. 4, pp. 525-556.

Affiliated with

Also associated with

Rating

Method for making stable, extracellular tyrosinase and synthesis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for making stable, extracellular tyrosinase and synthesis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for making stable, extracellular tyrosinase and synthesis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1503699