Synthetic herpes simplex virus promoters

Details Synthetic herpes simplex virus promoters Synthetic herpes simplex virus promoters

1994-10-31

1997-06-24

Guzo, David

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351721, 4351783, 4353201, 536 231, 536 241, C12P 2100, C12N 1586, C07H 2104

Patent

active

056416512

ABSTRACT:
The present invention relates to synthetic herpes simplex virus (HSV) promoters which are constructed by operatively linking the 5' nontranscribed domain of an HSV .alpha. gene to a fragment containing the transcription initiation site and the 5' transcribed noncoding region from an HSV .gamma. gene. Synthetic promoters of the invention that are operatively linked to heterologous genes, inserted into HSV genomes and used to generate live virus are useful for expressing polypeptides encoded by the heterologous genes in appropriate host cells. The synthetic promoters direct transcription of the heterologous genes constitutively throughout the reproductive cycle of the virus at a high cumulative level. The recombinant viruses of the invention can also be used as vaccines to present polypeptides against which a host will mount an immune response.

REFERENCES:
patent: 4859587 (1989-08-01), Roizman
Arsenakis et al., Methods in Molecular and Cellular Biology, 2:5-16(1990).
Honess et al., J. Virol., 14:8-19 (1974).
Honess et al., Proc. Natl. Acad. Sci. USA, 72:1276-1280 (1975).
Hummel et al., Virology, 148:337-343 (1986).
Meignier et al., J. Infect. Dis., 158:602-614 (1988).
Meignier et al., J Infect. Dis., 162:313-322 (1990).
Post et al., Mol. Cell, Biol., 2:233-240 (1982).
Post et al., Proc. Natl. Acad. Sci. USA, 77:4201-4205 (1980).
Post et al., Cell, 25:555-565 (1981).
Roizman et al., Eds., The Herpesviruses, vol. 3, Plenum Press, New York, 45-104 (1984).
Shih et al., Proc. Natl., Acad. Sci. USA, 81:5867-5870 (1984).
Banks et al. "Vaccination with the Immediate-Early Protein ICP47 of Herpes simplex Virus-Type 1 (HSV-1) Induces Virus-Specific Lymphoproliferation, but Fails to Protect Against Lethal Challenge", virology, vol. 200, pp. 236-245 (1994).
Wagner et al., Individual HSV Transcripts in Roizman et al. Eds., The Herpes Viruses, vol. 3, Plenum Press, New York, pp. 45-104 (1984).

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