Generation and selection of novel DNA-binding proteins and polyp

Details Generation and selection of novel DNA-binding proteins and polyp Generation and selection of novel DNA-binding proteins and polyp

1989-01-06

1992-03-17

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352523, 4353201, C12N 1563, C12N 1509, C12P 2100

Patent

active

050968157

ABSTRACT:
Novel DNA-binding proteins, especially repressors of gene expression, are obtained by variegation of genes encoding known binding protein and selection for proteins binding the desired target DNA sequence. A novel selection vector is used to reduce artifacts. Heterooligimeric proteins which bind to a target DNA sequence which need not be palindromic are obtained by a variety of methods, e.g., variegation to obtain proteins binding symmetrized forms of the half-targets and heterodimerization to obtain a protein binding the entire asymmetric target.

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