Assays, test kits and bacteria for detection of autoinducers

Details Assays, test kits and bacteria for detection of autoinducers Assays, test kits and bacteria for detection of autoinducers

1995-12-08

1998-06-02

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 6, C12Q 102

Patent

active

057597987

ABSTRACT:
The invention provides assays, kits and bacteria useful for detection of autoinducers. In a preferred aspect, the assay comprises 1) contacting a test sample suspected of containing an autoinducer with bacteria of the invention that are capable of producing an elevated amount of light in the presence of an exogenous autoinducer and that has at least two distinct genetic alterations that can each inhibit production of endogenous autoinducers; and 2) measuring the production of light. The sample will test positive for the presence of an autoinducer if a greater amount of light is produced relative to a control. The assays and kits have a variety of applications including use as an in vitro diagnostic for animal and plant disorders.

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