Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1997-09-08
1999-11-09
Carlson, Karen Cochrane
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530350, 530300, 514 12, C08H 100, C07K 1400
Patent
active
059817208
ABSTRACT:
Isolation of the salivary basic glycoproteins CON-1 and CON-2 in high purity and without substantial loss of activity was achieved in a method utilizing two denaturing steps which limit proteolysis. Cloning of CON-1 and CON-2 from the PRB2 and PRB1 genes also provides a recombinant source of the proteins, which are useful inhibitors of alpha-glucosidases in the treatment of diabetes and in preventing retroviral infection. Subfragments of CON-1 and CON-2 having alpha-glucosidase inhibitory activity are identified which can be prepared synthetically in commercial-scale quantities.
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Azen Edwin A.
Pan David
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