Oxidized phospholipid degrading enzyme and gene thereof

Details Oxidized phospholipid degrading enzyme and gene thereof Oxidized phospholipid degrading enzyme and gene thereof

1994-08-03

1998-12-15

Weber, Jon P.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435197, C12N 920, C12N 918

Patent

active

058495576

ABSTRACT:
An oxidized phospholipid degrading enzyme is provided, which plays an important role in the oxygen stress preventive mechanism in organisms. The enzyme hydrolyzes a 1-acyl-2-.omega.-carboxyfatty acid acyl-3-phosphatidylcholine as a substrate at the 2-ester bond thereof to form 1-acyl-2-lyso-3-phosphatidylcholine. The activity of the enzyme is slightly enhanced by 4 mM calcium chloride. The molecular mass of the enzyme is 95.+-.5 kDa (by gel filtration). The enzyme is composed of three subunits whose molecular masses have been found to be 29 kDa, 30 kDa and 45 kDa, respectively, by SDS-polyacrylamide electrophoresis. A gene coding the enzyme is also provided. This gene is important for the synthesis of the enzyme by genetic engineering.

REFERENCES:
patent: 5466595 (1995-11-01), Jones et al.
patent: 5519003 (1996-05-01), Mochley-Rosen et al.
Miwa et al. (1988) J. Clin. Invest., 82, "Characterization of Serum Platelet-Activating Factor (PAF) Acetylhydrolase", pp. 1983-1991.
Yanoshita et al. (1988) J. Biochem., 103(5), "Hydrolysis of Platelet Activating Factor and its Methylated Analogs by Acetylhydrolases", pp. 815-819.
Stafforini et al. (1993) J. Biol. Chem., 268(6), "The Platelet-Activating Factor Acetylhydrolase from Human Erythrocytes", pp. 3857-3865.
Banerjee et al. (1994) Science, 263, "inhA, a Gene Encoding a Target for Isoniazid and Ethionamide in Mycobacterium tuberculosis", pp. 227-230.
Tjoelker et al. (1995) Nature, 374, "Anti-Inflammatory Properties of a Platelet-Activating Factor Acetylhydrolase", pp. 549-553.
Bazan (1995) Nature, 374, "A Signal Terminator", pp. 501-502.
Journal of Biological Chemistry, vol. 268, No. 25, pp. 18748-18753, Sep. 5, 1993, Mitsuharu Hattori, et al., "Purification and Characterization of Bovine Brain Platelet-Activating Factor Acetylhydrolase".
Nature, vol. 370, pp. 216-218, Jul. 21, 1994, Mitsuharu Hattori et al., "Miller-Dieker Lissencephaly Gene Encodes a Subunit of Brain Platelet-Activating Factor".
Nature, vol. 364, pp. 717-721, Aug. 19, 1993, Orly Reiner, et al., "Isolation of a Miller-Dieker Lissencephaly Gene Containing G Protein Beta-Subunit-Like Repeats".
The Journal of Biological Chemistry, vol. 269, No. 37, pp. 23150-23155, Sep. 16, 1994, Mitsuharu Hattori, et al., "The Catalytic Subunit of Bovine Brain Platelet-Activating Factor Acetylhydrolase is a Novel Type of Serine Esterase".
Biochemical and Biophysical Research Communications, vol. 214, No. 1, pp. 180-187, Sep. 05, 1995, Hideki Adachi, et al., CDNA Cloning of Human Cytosolic Platelet-Activating Factor Acetylhydrolase Gamma-Subunit and its MRNA Expression in Human Tissues.
The Journal of Biological Chemistry, vol. 270, No. 52, pp. 31345-31352, Dec. 29, 1995, Mitsuharu Hattori, et al., "Cloning and Expression of a CDNA Encoding the Beta-Subunit (30-KDA Subunit) of Bovine Brain Platelet-Activating Factor Acetylhydrolase".
Sevanian et al. (1981) Lipids, 16(11), "Metabolism of Epoxidized Phosphatidylcholine by Phospholipase A.sub.2 and Epoxide Hydrolase", pp. 781-789.

Affiliated with

Also associated with

Rating

Oxidized phospholipid degrading enzyme and gene thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Oxidized phospholipid degrading enzyme and gene thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oxidized phospholipid degrading enzyme and gene thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1457010