Purification and characterization of alkaline phosphates from Th

Details Purification and characterization of alkaline phosphates from Th Purification and characterization of alkaline phosphates from Th

1997-02-25

1999-11-09

Witz, Jean C.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435196, A61K 3846

Patent

active

059808900

ABSTRACT:
This invention relates in general to alkaline phosphatase and, in particular, to alkaline phosphatase from a hyperthermophilic microorganism. The invention also relates to a method of purifying a hyperthermophilic alkaline phosphatase.

REFERENCES:
patent: 4030995 (1977-06-01), Starkweather
patent: 4656128 (1987-04-01), Chlebowski et al.
patent: 4659666 (1987-04-01), May et al.
patent: 4720458 (1988-01-01), Sullivan et al.
patent: 5264098 (1993-11-01), Chevigne
Bradford, Marion M., "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding", Analytical Biochemistry, 72: 248-254 (1976).
Coolbear, et al., "The Enzymes from Extreme Thermophiles," Advances in Biochemical Engineering Biotechnology, 45:77-78 (1992).
Dorn, Gordon L., "Purification and Characterization of Phosphatase I from Aspergillus nidulans", J. Biol. Chem., 243 No. 12: 3500-3506 (1968).
Fernley, H. N., "Mammalian Alkaline Phospatases", The Enzymes, Academic Press, New York vol. IV: 417-447 (1971).
Garen, et al., "A Fine-Structure Genetic and Chemical Study of the Enzyme Alkaline Phosphatase of E. coli", Biochem. Biophys. Acta, 38:470-483 (1960).
Glenn, et al., "Sporulation in Bacillus subtilis 168; Comparison of Alkaline Phosphatase from Sporulating and Vegetative Cells", Biochem. J., 123: 129-138 (1971).
Glew, et al., "Studies on the Extracellular Alkaline Phosphatase of Micrococcus sodonensis", J. Biol. Chem., 246: 1566-1574 (1971).
Gottesman, et al., "Kinetic Properties of Cobalt Alkaline Phosphatase", Biochemistry, 8 No. 9: 3776-3783 (1969).
Hartog, et al., "An Alkaline Phosphatase from Thermus sp Strain Rt41A," Int. J. Biochem., 24(10):1657-1660 (1992).
Jablonski, et al., "Preparation of Oligodeoxynucleotide-Alkaline Phosphatase conjugates and Their Use as Hybridization Probes", Nucleic Acid Research, 14 No. 15:6115-6128 (1986).
Janeway et al., "Magnesium in the Active Site of Escherichia coli Alkaline Phosphatase is Important for Both Structural Stabilization and Catalysis", Biochemistry, 32: 1601-1609 (1993).
Jannasch, et al., "Thermotoga neapolitana sp. nov. of the extremely thermophilic, eubacterial genus Thermotoga," Arch. Microbiol., 150:103-104 (1988).
Kim, et al., "Reaction Mechanism of Alkaline Phosphatase Based on Crystal Structures: Two-metal Ion Catalysis", J. Mol. Biol., 218: 449-464 (1991).
Laemmli, et al., "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4", Nature, 227: 680-685 (1970).
McComb, et al., "Alkaline Phosphatase", Plenum Press, New York, 27-51; 525-526; 206-207 (1979).
Say, et al., "Alkaline Phosphatase from Rat Osseous Plates: Purification and Biochemical Characterization of a Soluble Form", Biochimica et Biophysica Acta, 1074: 256-262 (1991).
Spencer, et al., "Effect of Colbalt on Synthesis and Activation of Bacillus licheniformis Alkaline Phosphatase", J. Bacteriology, 145 No. 2:926-933 (1981).
Vallee, et al., "New Perspective on Zinc biochemistry: Cocatalytic Site in Multi-Zinc Enzymes", Biochemistry, 32 No. 26, 6493-6495 (1993).
Wachsmuth, et al., "Alkaline Phosphatase from Pig Kidney", Biochem. J., 141:273-282 (1974).
Yasuura, et al., "Partial Purification of Alkaline Phosphatase form Bovine Polymorphonuclear Neutrophils and Some Properties", Comp. Biochem. Physiol. 82B No. 4: 587-593 (1985).
Yasuura, et al., "Characterization of Alkaline Phosphatase from Bovine Polymorphonuclear Neutrophils", Comp. Biochem. Physiol. 82B No. 4: 595-598 (1985).

Affiliated with

Also associated with

Rating

Purification and characterization of alkaline phosphates from Th does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification and characterization of alkaline phosphates from Th, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification and characterization of alkaline phosphates from Th will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1451800