Microdroplet test apparatus

Details Microdroplet test apparatus Microdroplet test apparatus

1983-09-13

1986-07-08

Warden, Robert J.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435 33, 435292, 435294, 435298, 435299, 435300, 435293, 435810, 436180, 436809, 422 73, 422 99, 422102, 350536, 356244, 356246, C12Q 120, C12M 126, C12M 132, C12M 128

Patent

active

045993157

ABSTRACT:
A microdroplet test apparatus for use in HLA typing. The apparatus is an improvement upon the well known Terasaki tray which is in use for determining HLA antigens by measuring lymphocyte cytotoxicity. The apparatus includes a tray having a plurality of microtest wells wherein the sides of the microtest wells are designed to promote localization of lymphocytes or other cells being tested at the microtest well bottom. The apparatus further includes a cover having either rods or tubes which extend down into the test solution present in the microtest well to thereby control the amount of test solution which is present in the cylindrical optical view path through which the test solution is viewed by microscope for test result measurements. This allows control of the amount of test solution in the optical view path in order to reduce the amount of solution which must be looked through when cells are being counted or alternatively, increasing the amount of test solution present in the view path in order to maximize detection when a colored reaction product in the test solution is being measured.

REFERENCES:
patent: 2956931 (1960-10-01), Goldberg
patent: 3912596 (1975-10-01), Haque et al.
patent: 4057470 (1977-11-01), Schrof
patent: 4278437 (1981-07-01), Haggar
patent: 4294924 (1981-10-01), Pepicelli et al.
patent: 4324859 (1982-04-01), Saxholm
patent: 4358908 (1982-11-01), Song
patent: 4432642 (1984-02-01), Tolles
patent: 4483925 (1984-11-01), Noack
Terasaki et al., Microdroplet Testing for HLA-A, -B, -C and -D, Antigens, Am. Journal of Clin. Path., vol. 69, No. 2, Feb. 78.

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