Method of determining polyamine content of biological fluids

Details Method of determining polyamine content of biological fluids Method of determining polyamine content of biological fluids

1982-09-15

1986-04-15

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435803, C12Q 152

Patent

active

045827940

ABSTRACT:
This application describes a method of determining the amount of organic polyamine in an aqueous solution to be assayed, which comprises (1) incubating a measured volume of said solution with a solution containing a protein with glutamine residues and a small, known amount of radiolabeled polyamine, in the presence of transglutaminase, (2) placing aliquots of the incubated solution on thin layer chromatographic strips treated with aqueous acid, (3) treating said strips with dilute aqueous acid solution to cause ascending chromatography, (4) cutting from the strips the sections where the aliquots had been applied thereto, (5) counting the radioactive scintillations of said sections, (6) carrying out steps (1) through (5) with the same volume of a standard solution containing a known amount of said polyamine, and (7) calculating the amount of polyamine in the solution to be assayed from the scintillation count made in the test procedure thereon in comparison to the scintillation count made in the test procedure conducted on the standard solution containing the known amount of said polyamine.

REFERENCES:
Haddox et al., P.N.A.S. USA, 78(3):1712-1716 (Mar. 1981).

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