Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1982-12-30
1986-04-15
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C12Q 170
Patent
active
045827877
ABSTRACT:
A method is disclosed for testing a patient to determine a predisposition to lung cancer and neurofibromatosis. The method comprises the steps of carrying out a biopsy on each patient of a first group of patients known to have a cancer or a cancer with a known hereditary component such as neurofibromatosis, the biopsy including the removal of a skin sample from each patient. The removed skin samples are prepared for in vitro testing thereof, initially to prove contamination is not present. In vitro testing is carried out to determine whether viral transformation of cultures established from the skin samples exists. Portions of Kirsten murine sarcoma virus are prepared and added to the uncontaminated cultured skin cells. The skin cells treated with the Kirsten murine sarcoma virus are incubated and a viral assay is performed to determine which skin cultures have been transformed due to the treatment with Kirsten murine sarcoma virus. The foregoing steps are performed relative to a second group of cancer and neurofibromatosis-free patients. An index is composed from the results of the assays performed on the first and second groups of patients. The foregoing steps are repeated relative to a subsequent patient, the predisposition to lung cancer and/or neurofibromatosis of which is unknown and a comparison of the results obtained from the assay relative to the subsequent patient is compared with the index to determine whether a predisposition to lung cancer and neurofibromatosis exists relative to the subsequent patient.
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