Limulus lysate of improved sensitivity and preparing the same

Details Limulus lysate of improved sensitivity and preparing the same Limulus lysate of improved sensitivity and preparing the same

1977-08-12

1978-08-15

Padgett, Benjamin R.

Compositions

Nonreactive analytical, testing, or indicating compositions

23230B, 1951035R, 424 2, 424 12, 424 95, 424101, C09K 300, G01N 3100, C12K 100

Patent

active

041070771

ABSTRACT:
Limulus lysate of improved sensitivity to endotoxin is prepared by withdrawing hemolymph from Limulus polyphemus (horseshoe crab), preparing a lysate by lysing the amoebocytes obtained from the hemolymph, treating the lysate with an amount of an organic solvent sufficient to precipitate a clotting inhibitor within the lysate and isolating the organic solvent treated lysate. In another embodiment, the sensitivity of the lysate is further increased by adding a salt of calcium, magnesium, manganese, thereto.

REFERENCES:
patent: 3915805 (1975-10-01), Levin
patent: 3944391 (1976-03-01), Harris et al.
patent: 3954663 (1976-05-01), Yamamoto et al.
patent: 4038029 (1977-07-01), Teller et al.
patent: 4038147 (1977-07-01), Reno
Yin, E., et al., Biochim. Biophys. Acta., vol. 261, pp. 284-289 (1972).
Hochstein, D., et al., Bull. Parenteral Drug Assc., vol. 27, No. 3, pp. 139-148 (1973).
Young, N., et al., J. Clin. Invest., vol. 51, pp. 1790-1797 (1972).
Nachum, R., et al., N. Engl. J. Med., vol. 289, pp. 931-934 (1973).

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