Element for electrophoresis containing polyacrylamide gel membra

Details Element for electrophoresis containing polyacrylamide gel membra Element for electrophoresis containing polyacrylamide gel membra

1985-05-14

1988-04-12

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, 4284793, B01D 5702

Patent

active

047372583

ABSTRACT:
An element for electrophoresis suitably employable for electrophoresis of biopolymers such as proteins, as well as for determination of base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic support, an adhesive layer containing a cellulose derivative, and an electrophoresis medium layer comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.

REFERENCES:
patent: 4415428 (1983-11-01), Nochumson et al.
patent: 4548869 (1985-10-01), Ogawa et al.

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