Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Having -c- – wherein x is chalcogen – bonded directly to...
Patent
1998-01-26
1998-12-29
Richter, Johann
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Having -c-, wherein x is chalcogen, bonded directly to...
435118, 548437, 548450, 548449, A01N 4336, C12P 1716, C07D20982, C07D20994
Patent
active
058542760
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to WF16616 substance, a process for producing the same and use thereof. WF16616 substance is a novel substance which is isolated from a culture broth of microorganisms, especially imperfect fungi. This substance exhibits an excellent physiological activity, and is effective as an antimicrobial agent for humans and other animals, especially as an agent for preventing or treating diseases caused by microorganisms, molds, other fungi or protozoans, such as an antifungal agent and an agent for Pneumocystis carinii pneumonia.
PRIOR ART
A variety of antimicrobial substances have been developed so far, but few of them are satisfactory with respect to effectiveness, antimicrobial spectrum, safety, production cost and the like. Therefore, the development of novel substances has been in high demand in the art.
Problems to be Solved by the Invention
The present invention has been conducted to meet such a demand in the art and to develop a novel substance that exhibits an outstanding physiological activity.
Means Taken for Solving the Problems
The present inventors have conducted various investigations to achieve the above-mentioned purpose. Consequently, they have focussed their attention on fermentative products of microorganisms, and have earnestly screened various microorganisms. As a result, they have newly found that an extract obtained from a culture broth of No. 16616 strain which was newly separated from a soil sample collected in Takato-cho, Nagano-ken exhibits an excellent antimicrobial activity, especially an antifungal activity. They have further studied the physico-chemical properties of this substance in detail, and have identified that this substance is a novel substance which has been so far unknown. They have named this substance "WF16616". Upon further investigations, they have established an industrial process for producing the same, and have completed the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a chart showing a .sup.1 H nuclear magnetic resonance spectrum of a salt (which is considered to be a sodium salt, a potassium salt, an ammonium salt or a mixture thereof) of WF16616 substance.
FIG. 2 is a chart showing a .sup.13 C nuclear magnetic resonance spectrum of a salt (which is considered to be a sodium salt, a potassium salt, an ammonium salt or a mixture thereof) of WF16616 substance.
WF16616 substance of the present invention has been found to be represented by the following structural formula (1) upon studies on physico-chemical properties and the like which will be later described. ##STR2##
WF16616 substance of the present invention is produced by, for example, No. 16616 strain which is a filamentous fungus separated from a soil sample collected in Takato-cho, Kamiina-gun, Nagano-ken. This microorganism is restrictedly grown on various media and forms a yellowish white colony. Further, on a variety of media No. 16616 strain forms an anamorph consisting of a conidiophore having a verticillate phialide and a sticky conidial mass. The phialide consists of a swollen base and an elongated stem. In view of these morphological characteristics, the microorganism is identied to belong to the genus Tolypocladium W. Gams (Gams, W: Persoonia, 6:185-191, 1971) which is an imperfect fungus. The mycological properties thereof are described below.
The cultural properties of this microorganism in a variety of agar media are summed up in Tables 1 and 2 below. It was restrictedly grown through incubation on a potato dextrose agar medium, and was spread in a diameter of from 1.5 to 2.0 cm after incubation at 25.degree. C. for 2 weeks. The colony surface was felty or cottony, and somewhat raised. The central portion and the peripheral portion of the colony were white to yellowish white, and the portion therebetween was grayish brown. The reverse thereof was olive brown and yellowish white. The conidial structures were abundantly observed. The colony was restrictedly grown on a corn meal agar medium also, and was spread in a di
REFERENCES:
Agathos, S.N., Enhancement of Cyclosporin Production in a Tolypocladium Inflatum Strain After Epichlorohydrin Treatment, Journal of Biotechnology, 13 73-78, Dec. 1990.
Hashimoto Seiji
Hatanaka Hiroshi
Kino Toru
Okudaira Terumi
Tsurumi Yasuhisa
Fujisawa Pharmaceutical Co. Ltd.
Keating Dominic
Richter Johann
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