Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1997-01-06
1998-12-29
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435832, 424 93461, C12N 120, A01N 6300
Patent
active
058540539
ABSTRACT:
A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
REFERENCES:
patent: 5080897 (1992-01-01), Gonzalez, Jr. et al.
patent: 5204237 (1993-04-01), Gaertner et al.
patent: 5441884 (1995-08-01), Baum
Gleave et al., "Identification of An Insecticidal Crystal Protein from Bacillus thuringiensis DSIR517 with Significant Sequence Differences from Previously Described Toxins," J. Gen. Microbiol., 138:55-62 (1992).
Turner et al., "Stability of the .delta.-Endotoxin Gene from Bacillus thuringiensis subsp. kurstaki in a Recombinant Strain of Clavibacter xyli subsp. cynodontis," Appl. Environ. Microbiology., 57:3522-3528 (1991).
Smulevitch et al., "Nucleotide Sequence of a Novel .delta.-Endotoxin Gene cryIg of Bacillus thuringiensis ssp. galleriae," FEBS Lett., 293:25-28 (1991).
Chambers et al., "Isolation and Characterization of a Novel Insecticidal Crystal Protein Gene from Bacillus thuringiensis subsp. aizawai," J. Bacteriol., 173:3966-3976 (1991).
Perlak et al., "Modification of the Coding Sequence Enhances Plant Expression of Insect Control Protein Genes," Proc. Natl. Acad. Sci. USA, 88:3324-3328 (1991).
Macaluso et al., "Efficient Transformation of Bacillus thuringiensis Requires Nonmethylated Plasmid DNA," J. Bacteriol., 173:1353-1356 (1991).
Von Tersch et al., "Insecticidal Toxins from Bacillus thuringiensis subsp. kenyae: Gene Cloning and Characterization and Comparison with B. thuringiensis subsp. kurstaki CryIA(c) Toxins," Appl. Environ. Microbiology, 57:349-358 (1991).
Visser et al., "A Novel Bacillus thuringiensis Gene Encoding a Spodoptera exigua-specific Crystal Protein," J. Bacteriol., 172:6783-6788 (1990).
Mettus et al., "Expression of Bacillus thuringiensis .delta.-Endotoxin Genes During Vegetative Growth," Appl. Environ. Microbiology, 56:1128-1134 (1990).
Hodgman et al., "Models for the Structure and Function of the Bacillus thuringiensis .delta.-Endotoxins Determined by Compilational Analysis," J. DNA Sequencing and Mapping, 1:97-106 (1990).
Hofte et al., "Insecticidal Crystal Proteins of Bacillus thuringiensis," Microbiol. Rev., 53:242-255 (1989).
Sanchis et al., "Nucleotide Sequence and Analysis of the N-terminal Coding Region of the Spodoptera-active .delta.-Endotoxin Gene of Bacillus thuringiensis aizawai 7.29," Molecular Microbiol., 3:229-238 (1989).
Masson et al., "Nucleotide Sequence of a Gene Cloned from Bacillus thuringiensis subspecies entomocidus Coding for an Insecticidal Protein Toxic for Bombyx mori," Nucl. Acids Res., 17:446 (1989).
Haider et al., "Nucleotide Sequence of a Bacillus thuringiensis aizawai IC1 Entomocidal Crystal Protein Gene," Nucl. Acids Res., 16:10927 (1988).
Honee et al., "Nucleotide Sequence of Crystal Protein Gene Isolated from B. thuringiensis subspecies entomocidus 60.5 Coding for a Toxin Highly Active Against Spodoptera species," Nucl. Acids. Res., 16:6240 (1988).
Brizzard et al., "Nucleotide Sequence of an Additional Crystal Protein Gene Cloned from Bacillus thuringiensis," Nucl. Acids. Res., 16:2723-2724 (1988).
Shimizu et al., "Cloning and Expression in Escherichia coli of the 135-kDa Insecticidal Protein Gene from Bacillus thuringiensis subsp. aizawai IPL7," Agric. Biol. Chem., 52:1565-1573 (1988).
Visser et al., "Genes from Bacillus thuringiensis entomocidus 60.5 Coding for Insect-specific Crystal Proteins," Mol. Gen. Genet., 212:219-224 (1988).
Fischoff et al., "Insect Tolerant Transgenic Tomato Plants," Bio/Technology, 5:807-813 (1987).
Oeda et al., "Nucleotide Sequence of the Insecticidal Protein Gene of Bacillus thuringiensis Strain aizawai IPL7 and its High-level Expression in Escherichia coli," Gene, 53:113-119 (1987).
Hefford et al., "Sequence of a Lepidopteran Toxin Gene of Bacillus thuringiensis subsp. kurstaki NRD-12," J. Biotechnology, 6:307-322 (1987).
Kondo et al., "Cloning and Nucleotide Sequencing of Two Insecticidal .delta.-Endotoxin Genes from Bacillus thuringiensis var. kurstaki HD-1 DNA," Agric. Biol. Chem., 51:455-463 (1987).
Geiser et al., "The Hypervariable Region in the Genes Coding for Entomopathogenic Crystal Proteins of Bacillus thuringiensis: Nucleotide Sequence of the kurhdl gene of subsp. kurstaki HD1," Gene, 48:109-118 (1986).
Obukowicz et al., "Integration of the Delta-endotoxin Gene of Bacillus thuringiensis Into the Chromosome of Root-colonizing Strains of Pseudomonads Using Tn5," Gene, 45:327-331 (1986).
Wabiko et al., "Bacillus thuringiensis Entomocidal Protoxin Gene Sequence and Gene Product Analysis," DNA, 5:305-314 (1986).
Hofte et al., "Structual and Functional Analysis of a Cloned Delta Endotoxin of Bacillus thuringiensis berliner 1715," Eur. J. Biochem., 161:273-280 (1986).
Schnepf et al., "The Amino Acid Sequence of a Crystal Protein from Bacillus thuringiensis Deduced from the DNA Base Sequence," J. Biol. Chem., 260:6264-6272 (1985).
Adang et al., "Characterized Full-length and Truncated Plasmid Clones of the Crystal Protein of Bacillus thuringiensis subsp. kurstaki HD-73 and Their Toxicity to Manduca sexta," Gene, 36:289-300 (1985).
Shibano et al., "Nucleotide Sequence Coding for the Insecticidal Fragment of the Bacillus thuringiensis Crystal Protein," Gene, 34:243-251 (1985).
Carlton et al., "Plasmids and Delta-Endotoxin Production in Different Subspecies of Bacillus thuringiensis," Molecular Biology of Microbial Differentiation, pp. 246-252, J.A. Hoch and P. Setlow, ed., American Society for Microbiology, Washington, DC (1985).
King et al., "Heliothis Virescens,"in Handbook of Insect Rearing, vol. II, pp. 323-328, P. Singh and R.F. Moore (eds.), Elsevier Science, Amsterdam (1985).
Kaiser et al., "Amphiphilic Secondary Structure: Design of Peptide Hormones," Science, 223:249-255 (1984).
Gonzalez, Jr., et al., "Transfer of Bacillus thuringiensis Plasmids Coding for .delta.-endotoxin Among Strains of B. thuringiensis and B. cereus," Proc. Natl. Acad. Sci. USA, 79:6951-6955 (1982).
Donovan William P.
Gonzalez, Jr. Jose M.
Ecogen Inc.
Prouty Rebecca E.
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