Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1989-04-10
1991-08-27
Moskowitz, Margaret
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
530412, 530419, 435 697, C07K 324, C07K 1512, C12N 1518
Patent
active
050434306
ABSTRACT:
A process for the solubilization of a protein obtained via heterologous expression comprises the step of bringing the insoluble protein produced in transformed host cells into contact with an aqueous acid solution in the presence of a protein denaturant. The process also preferably comprises the further steps of elevating the pH value of the solution, by addition of alkali substance, and thereafter lowering the pH to obtain a biologically-active protein.
REFERENCES:
Greene et al., Proc. Natl. Acad. Sci. USA, "Establishment of a Noradrenergic Clonal Line of Rat Adrenal Pheochromocytoma Cells which Respond to Nerve Growth Factor", vol. 73, No. 7, pp. 2424-2428, Jul. 1976.
Biotechnology, vol. 7, pp. 1141-1149, Catherine H. Schein, "Production of Soluble Recombinant Proteins in Bacteria", Nov. 1989.
Farman Keith L.
Moskowitz Margaret
Tosoh Corporation
LandOfFree
Process for treating insoluble heterologous protein does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for treating insoluble heterologous protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for treating insoluble heterologous protein will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1415077