Method of producing plant cell lines and plant hybrids

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352404, 43524045, 800200, 800205, 47 58, C12N 504, A10H 400, A10H 100

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050432826

ABSTRACT:
A method is disclosed for producing, in a single culture, plant cells that contain a self incompatibility (SI) determinant and plant cells that do not. A selected line is crossed with a line that is homozygous for an SI determinant, and pollen or microspores from the resulting progeny are cultured to obtain haploid cells lacking that determinant. These cells can be used, respectively, to produce sister lines that, when crossed, yield SI-heterozygous foundation seed for producing a hybrid.

REFERENCES:
Ziemborska et al. (1986). U. of Guelph-Crucifer Genetics Coop. 1986 (70) (Abstract relied on).
Hoffman et al. (1982). Theor. Appl. Genet. 61:225-232.
Downey et al. (1987). My Principles of Cultivation Development, vol. 2. Ed. Walter R. Feler. MacMillan Pub. Co. N.Y. p. 473.
Gowers, "Eucarpia Cruciferae Newsletter" vol. 5; p. 15 (1980).
Ziemborska, "Anther Culture of Rutabaga-Rape Hybrids (Brassica Napus SSP. Rapifera . . . "(1986) pp. 1-3 and 105-180.
Thompson, "Breeding Winter Oilseed Rape, Brassica Napus" In Advances in Applied Biology; vol. 7, pp. 31-35 (1983).
Gowers, "Self-Incompatibility in Brassica Napus" in Proceedings-Eucarpia Cruciferae 1979 Conference.
Euphytica 24 (1975) pp. 537-541 "Methods of Producing F.sub.1 Hybrid Swedes"; S. Gowers.

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