Method of regulating expression of a foreign gene by controlling

Details Method of regulating expression of a foreign gene by controlling Method of regulating expression of a foreign gene by controlling

1988-02-17

1991-08-27

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 435 711, 435 91, 435170, 435232, 43525253, 4353201, 435849, 536 27, 935 6, 935 8, 935 9, 935 22, 935 29, 935 33, 935 38, 935 39, 935 59, 935 60, 935 61, 935 66, 935 73, C12N 121, C12N 1552, C12N 1568, C12N 1570, C12N 988

Patent

active

050432770

ABSTRACT:
Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured at a temperature 40.degree. C. or over so that the expression of said desired foreign gene was suppressed. This E. coli (i.e., transformant) was cultured at 40.degree. C. or over in a first process to suppress the expression of the foreign gene and to support sufficient cell growth and thereafter below 40.degree. C. in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.

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