Amplification of nucleic acid sequences using oligonucleotides o

Details Amplification of nucleic acid sequences using oligonucleotides o Amplification of nucleic acid sequences using oligonucleotides o

1989-04-27

1991-08-27

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435810, 436501, 436 94, 935 77, 935 78, C12P 1934, C12Q 168, G01N 33566, G01N 3348

Patent

active

050432729

ABSTRACT:
According to this invention, a process for substantially amplifying template nucleic acid present in a sample is described, wherein said amplification may be performed without prior knowledge of specific sequences, which process comprises apposition of random oligonucleotide primers to said template nucleic acid under conditions such that extension products of said primers are synthesized which are complementary to said template nucleic acid.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
Maniatis et al., Molecular Cloning: A Lab. Manual, Cold Spring, Harbor Lab., N.Y. (1982), pp. 129 & 131.
Shibata et al., J. Exp. Med. 167(1):225-230 (1988).
Noonan, K. E. et al., Nucl. Acids Res., 16:10366 (1988).
Feinberg, A. P. et al., Anal. Biochem., 132:6-13 (1983).
Liang, W. et al., Nucl. Acids Res., 16:3579 (1988).
Mullis, K. et al., Cold Spring Harb. Symp. Quant. Biol., 51:263-273 (1986).
Loh et al., Science, 243:217-200 (1988).
Landegren, U., et al., Science, 242:229-237 (1988).
Mullis, K. B. et al., Meth Enzymol., 155:335-350 (1987).
Marx, J. L., Science, 240:1408-1410 (1988).
"Amplifying DNA by the Magic of Numbers", Science, 233:159 (1986).

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