Production of acidic FGF protein

Details Production of acidic FGF protein Production of acidic FGF protein

1994-04-04

1995-03-07

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352402, 435 691, 4353201, 43525233, C12N 1500, C12N 500

Patent

active

053957563

ABSTRACT:
An expression vector comprises a cDNA sequence encoding an aFGF protein and a T7 promoter upstream therefrom. The vector is useful for transforming host cells and expressing the aFGF gene.

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Rosenberg, et al., "Vectors for selecive expression of cloned DNAs" by T7 RNA polymerase, Gene 56, 15-135 (1987).
Gimenez-Gallego, G. et al. Biochem. Biophys. Res. Commun. vol. 138, 611 (1986).
Brew, K. et al. (Eds.) ICSU Short Report 8, Advances in Gene Technology: Protein Engineering and Production; IRL Press; Washington, DC, p. 110, 1988.
Barr, P. et al., J. Biological Chemistry, 263, 16471 (1988).
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Watanabe, T. et al., Folia Endocrinologica Japonica vol. 66, No. 4, p. 359, Title 276.
Watanabe, T. et al., Molecular Endocrinology vol. 4, No. 6, pp. 869-879 (1990).

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