Monoclonal antibodies to crystal protein of Bacillus thuringiens

Details Monoclonal antibodies to crystal protein of Bacillus thuringiens Monoclonal antibodies to crystal protein of Bacillus thuringiens

1987-05-18

1990-07-31

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 7021, 4351722, 435948, 435832, 530387, 530809, 530825, 436548, 935104, 935108, 935110, 935 93, C12N 520, C07K 1528

Patent

active

049450573

ABSTRACT:
Murine hybridomas are disclosed which were constructed by fusing spleen cells from BALB/c mice immunized with soluble crystal protein from Bacillus thuringiensis subsp. israelensis (B.t.i.) to the murine myeloma cell line SP2/0-AG14. An ELISA (enzyme-linked immunosorbent assay) method for detection of antibodies specific for crystal protein of B.t.i. was modified to produce 100- to 1,000-fold increased sensitivity and was used to identify hybridomas secreting monoclonal antibody specific for the B.t.i. crystal protein. Analysis of the hybridoma culture supernatant fluid indicated production of monoclonal IgG3 antibodies, specific for the 68,000 dalton protein presumed to be the insecticidal delta-endotoxin of B.t.i.

REFERENCES:
Hurley, J. M. et al., "Separation of the Cytolytic and Mosguitocidal Proteins of Bacillus thuringiensis subsp. israeleusis", Biochem. Biophys. Res. Commun., 126(2): 961-965, Jan. 31, 1985.
Lee, S. G. et al., "Diversity of Protein Inclusion Bodies and Identification of Mosquitocidal Protein in Bacillus thuringiensis subsp. israelensis", Biochem. Biophys. Res. Commun., 126(2): 953-960, Jan. 31, 1985.
Armstrong, J. L. et al., "Delta Endotoxin of Bacillus thuringiensis subsp. israelensis", J. Bacteriol., 161(1): 39-46, Jan. 1985.
Temeyer, K. B. et al., "Production and Use of Monoclonal Antibodies Specific for Crystal Protein of Bacillus thuringiensis var. israelensis", J. Cell. Biochem., Suppl. 10C:92, Mar. 30-Apr. 12, 1986.
Huber-Lukac, M. et al., Experientia, 38, pp. 1103-1105, (1982).
Huber-Lukac, M. et al., American Society for Microbiology, 40:2, pp. 608-612, (May 1983).
Huber-Lukac, M. et al., Infection and Immunity, 54:1, pp. 228-232, (1986).
Temeyer, Kevin B., Applied and Environmental Microbiology, 47:5, pp. 952-955, (May 1984).
Zola, H. and D. Brooks, "Techniques for the Production and Characterization of Monoclonal Hybirdoma Antibodies", Monoclonal Hybridoma AntiBodies: Techniques and Applications, pp. 1-57.

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