Reagent used for determining factor VIII activity

Details Reagent used for determining factor VIII activity Reagent used for determining factor VIII activity

1992-01-10

1996-04-09

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435 696, 435975, 436 69, 530383, 530384, 424529, C12Q 156, C12P 2104, G01N 3353, A61K 3514

Patent

active

055061125

ABSTRACT:
To determine the factor VIII activity of a sample, the latter is reacted with a reagent containing thrombin, factor IXa.beta., phospholipids and calcium ions so as to form a factor VIIIa-factor IXa-phospholipid-calicum ion-containing complex. This complex is reacted with factor X so as to obtain activated factor X. The obtained factor Xa is reacted with chromogenic substrate so as to form a substance to be determined spectrophotometrically. Complex formation and activation of factor X are effected in one stage: The sample is mixed with a mixture of human thrombin, phospholipids, calcium ions, human factor IXa.beta., human factor X and, if desired, human factor XIa and human factor XIIa, as a complex-forming reagent.

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