Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1997-01-15
1999-06-29
Woodward, Michael P.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530402, 530410, 514 6, C07C10352, C07K 1300, A61K 3514
Patent
active
059170204
ABSTRACT:
Crosslinking reagents for protein modification are provided, which comprise four functional, leaving groups capable of reacting with amino acid residues on the protein chains, one pair at each end of a relatively rigid chemical spacer group comprising aromatic residues joined by amide linkages. Such crosslinking reagents react with hemoglobin to effect intramolecular crosslinking of two dimeric hemoglobin units to stabilize the tetramers, by use of the respective pairs of functional groups, and to produce dimers of such tetrameric units, i.e. bis-tetrameric, intramolecularly crosslinked and stabilized hemoglobin, to the exclusion of higher molecular weight hemoglobin species.
REFERENCES:
patent: 4857636 (1989-08-01), Hsia
patent: 5250665 (1993-10-01), Kluger et al.
patent: 5399671 (1995-03-01), Kluger et al.
patent: 5532352 (1996-07-01), Pliura et al.
K. Paal, R.T. Jones, & R.H. Kluger, J. Am. Chem. Soc. 118, 10380-10383.
He Huang, et al., "Multilinking Reactions Between Hemoglobin Tetramers in Solution and the Crystal", Artificial Cells, Blood Substitutes and Immobilization Biotechnology, vol. 22, No. 5, 1994, p. 106.
Zheng, Y., and Olsen, K.W., "Multilinking of Hemoglobin for Potential Blood Substitutes and Bioconjugates" (Meeting Abstract) Biophysicial Journal 1996, 70(2) Part 2, WP 390.
K. Paal et al.: A Site-Specific Tetrafunctional Reagent for Protein Modification: Cross-linked Hemoglobin With Two Sites for Further Reaction, Journal of the American Chemical Society, vol. 118, 1996.
Aharoni et al. Macromolecules 23(9): 2533-2549, 1990.
Aharoni et al. Macromolecules 22(8): 3361-3374, 1989.
Kluger et al. J. Am. Chem. Soc. 118(43): 10380-10383; Kluger et al. (1996a).
Kluger et al. J. Am. Chem. Soc. 118(37): 8782-8786; Kluger et al. (1996b).
Kluger et al. J. Am. Chem. Soc. 114(24): 9275-9280, 1992.
Delaney et al. Archives of Biochemistry and Biophysics 228(2): 627-638, 1984.
Kluger Ronald H.
Lock-O'Brien Jodi J.L.
Hirons Robert G.
Williams Jay F.
Woodward Michael P.
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