Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-09-15
1999-04-06
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 4351723, 43525233, 435194, C12Q 168
Patent
active
058916377
ABSTRACT:
A method of producing cDNA from mRNA is described in which the 5' end of mRNA is capped and introduced into a vector so that both the 5' and 3' ends become annealed to flanking sequences of the vector. Reverse transcriptase is then used to convert the mRNA into dscDNA, the reverse transcriptase being employed in vivo, in vitro or using a combination of these approaches. Preferably, the conversion of mRNA to dscDNA is carried out in a cell line transformed with a second vector producing the reverse transcriptase, the cell line supplying the other enzymes and materials needed for cDNA synthesis. Also described are applications of this method to construct and screen cDNA libraries and cell lines transformed with both vectors.
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Dreger Ginger R.
Genentech Inc.
Ketter James
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