Subtilisin analogs

Details Subtilisin analogs Subtilisin analogs

1987-04-10

1990-04-03

Mays, Thomas D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4351721, 4351723, 435220, 435221, 935 14, 935 74, 935 82, C12N 956, C12N 954, C12N 1500

Patent

active

049140310

ABSTRACT:
A class of subtilisin analogs suitable for admixture to cleaning compositions and having improved stability over naturally occurring Bacillus subtilisins are prepared by expressing a modified gene encoding the subtilisin analog in Bacillus subtilis. The subtilisin analogs are characterized as having a modified calcium binding site to improve calcium binding and either an Asn or a Gly replaced in any Asn-Gly sequences present in the subtilisin.

REFERENCES:
patent: 4760025 (1988-07-01), Estell et al.
Thomas et al., Nature, 318: 375, 1985.

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