Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1993-12-10
1996-02-20
Beisner, William H.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435240241, 4352891, 4352982, 4353041, 600 36, 623 1, C12N 506, C12M 304
Patent
active
054928265
ABSTRACT:
An apparatus and method for culturing living endothelial cells is provided. The apparatus comprises a cell growth container, which in a preferred embodiment is a synthetic graft, heat crimped at both ends. The apparatus further comprises a rotation container that may contain the cell growth container and is attached to a rotation apparatus. The apparatus further comprises fixturing apparatus, which in a preferred embodiment is a pair of o-ring members, that enhances a separated condition of the cell growth container within the rotation container. The method of the present invention comprises providing a culture that includes living cells and a growth medium, introducing the culture into a cell growth container, heat crimping the cell growth container to substantially seal the cell growth container, and positioning fixturing apparatus upon the external surface of the cell growth container. This assembly is inserted into a rotation container, which is rotated by a rotation apparatus.
REFERENCES:
patent: 3883393 (1975-05-01), Knazek et al.
patent: 4024020 (1977-05-01), Weiss et al.
patent: 4238568 (1980-12-01), Lynn
patent: 4283495 (1981-08-01), Lynn
patent: 4495288 (1985-01-01), Jarvis, Jr. et al.
patent: 4647539 (1987-03-01), Bach
patent: 4824787 (1989-04-01), Serkes et al.
patent: 4845038 (1989-07-01), Barr et al.
patent: 4871671 (1989-10-01), Errede et al.
patent: 4908013 (1990-03-01), Muller et al.
patent: 4912058 (1990-03-01), Mussi et al.
patent: 4962033 (1990-10-01), Serkes et al.
patent: 4988623 (1991-01-01), Schwarz et al.
patent: 5035708 (1991-07-01), Alchas et al.
patent: 5104802 (1992-04-01), Rhodes et al.
patent: 5171261 (1992-12-01), Noishiki et al.
Shunya S. et al., "Improved patency of collagen-impregnated grafts after in vitro autogenous endothelial cell seeding" J. Vas. Surg. 6(4):325-32 (1987).
van Oene, G. H., et al., "Smooth Muscle Cell Seeding Enhances Neo-Endothelialization" 1st Eur. Workshop Advanced Technologies in Vascular Surgery, Vienna 1986, pp. 160-166 (Karger, Basel 1987).
Zilla, P. et al., "Endothelial cell seeding of polytetrafluoroethylene vascular grafts in humans: A preliminary report", J. Vas. Surg. 6(6):535-41 (1987).
Kaehler, J. et al., "Precoating substrate and surface configuration determine adherence and spreading of seeded endothelial cells on polytetrafluoroethylene grafts", J. Vas. Surg., 9:535-541 (1989).
Zilla, P., et al., "Use of fibrin glue as a substrate for in vitro endothelialization of PTFE vascular grafts",; J. Surg., 105(4):515-522 (1989).
Gerlach, J., et al., "Endothelial Cell Seeding on Different Polyurethanes", J. Artif. Organs. 13(2):144-7 (1989).
Gerlach, J., et al., "Endothelial cell seeding on PTFE vascular prostheses using a standardized seeding technique", Intl. J Artif. Organs 12(4):270-5 (1989).
Budd, J. S., et al., "Attachment of indium-111 labelled endothelial cells to pretreated polytetrafluoroethylene vascular grafts", Br. J. Jurg., 76:1259-61 (1989).
James, N. L., et al., et al., "Endothelial Cell Seeding of Small Diameter Vascular Grafts", J. Artif. Organs 14(5):355-60 (1990).
Thomson, George J. L., et al., "Adult human endothelial cell seeding using expanded polytetrafluoroethylene vascular grafts: A comparison of four substrates" J. Surg. 109(1): 20-7 (1991).
Miyata, Etsuro, et al., "Delayed Exposure to Pulsatile Shear Stress Improves Retention of Human Saphenous Vein Endothelial Cells on Seeded ePTFE Grafts", J. Surg. Research 50(5):485-93 (1991).
Budd, J. S., et al., "Effects of two methods of endothelial cell seeding on cell retention during blood flow", Br. J. Surg. 78:878-882 (1991).
Newman, Kurt D., et al., "Quantification of Vascular Graft Seeding by Use of Computer-Assisted Image Analysis and Genetically Modified Endothelial Cells", J. Vas. Surg. 14(2): 140-6 (1991).
Borrelli Michael J. E.
Maupin Diane E.
Townsend Laurace E.
Beisner William H.
William Beaumont Hospital
LandOfFree
Apparatus and method for seeding endothelial cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Apparatus and method for seeding endothelial cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus and method for seeding endothelial cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1355583