Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters carbohydrate production in the plant
Patent
1998-04-13
1999-11-16
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters carbohydrate production in the plant
800287, 800288, 800298, 800320, 8003201, 435 697, 435 698, 435101, 435193, 435468, 536 234, 536 236, 536 237, 536 241, C12N 1582, C12N 1529, C12N 1531, C12N 1554, C12P 1904
Patent
active
059861738
ABSTRACT:
The present invention relates to a method for obtaining transgenic plants showing a modified fructan pattern as compared to non-transformed plants, comprising the steps of: a) preparing a DNA construct comprising one or more fructosyltransferase genes, or modified versions thereof, being operably linked to a promoter sequence active in plants and a terminator sequence active in plants; b) transforming a plant cell with the construct; and c) regenerating a transgenic plant from the transformed plant cell. Preferably, the 5' untranslated region of the fructosyltransferase gene is modified such that the expression of the fructosyltransferase is not negatively affected. The DNA construct may also comprise a targeting sequence upstream from the fructosyltransferase gene for directing the fructosyltransferase to a specific plant tissue or plant cell compartment. The fructosyltransferases produced in the transgenic plants change the fructan pattern of the plants, thus leading to different plant performance due to altered sink-source relations and yield, increased tolerance for drought, cold or other stresses, higher dry matter content, better taste and storability, and improved nutritional value. The plants are also suitable for use as raw material for fructan production.
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Ebskamp Michael Johannes Marcus
Smeekens Josephus Christianus Maria
Weisbeek Petrus Jacobus
Fox David T.
Stichting Scheikundig Onderzoek in Nederland
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