Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1998-04-24
1999-11-16
Crane, L. Eric
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 254, C07H 2100, C07H 2102, C07H 2104
Patent
active
059860855
ABSTRACT:
A batch process for obtaining polynucleotide fragments, such as dsDNA, having a selected size from a mixture of polynucleotide fragments including the steps of a) applying a solution of the mixture of polynucleotide fragments and a counterion agent to a binding medium having a hydrophobic surface; b) contacting the binding medium with a first stripping solvent and counterion agent, the first stripping solvent having a concentration of organic component sufficient to release from the binding medium all polynucleotide fragments having a size smaller than the selected size, and removing the first stripping solvent from the binding medium; and c) contacting the binding medium with a second stripping solvent having a concentration of organic component sufficient to release from the binding medium the polynucleotide fragments having the selected size, and removing the second stripping solvent from the binding medium. The binding medium can be organic polymer or inorganic particle beads. The mixture of polynucleotides can be the product of a PCR amplification. The binding medium can be contained within a column, a web or a container.
REFERENCES:
patent: 4906378 (1990-03-01), Hagen et al.
patent: 4997927 (1991-03-01), Blocker et al.
patent: 5098539 (1992-03-01), Shieh
patent: 5338448 (1994-08-01), Gjerde
patent: 5413762 (1995-05-01), Hirano et al.
patent: 5585236 (1996-12-01), Bonn et al.
patent: 5616701 (1997-04-01), Woodard et al.
patent: 5705628 (1998-01-01), Hawkins
Draper et al., "High-Performance Anion-Exchange Chromatography of Oligonucleotides," Analytical Biochemistry, 145(1), 47-56 (Feb. 15, 1985).
Huang et al., "Large-Scale Purification of Synthetic Oligonucleotides and Carcinogen-Modified Oligodeoxynucleotides on a Reverse-Phase Polystyrene (PRP-1)," Analytical Biochemistry, 190(1), 21-25 (Oct. 1990).
Furst et al., "Simultaneous Determination of Myocardial Nucleotides, Nucleosides, Purine Bases and Creatine Phosphate by Ion-Pair High-Performance Liquid Chromatography," 578(1), 39-44 (Jul. 1, 1992).
Vtorushina et al., "Study of Diastereomers of Non-Ionic Oligonucleotide Analogues. VI. Separation of Diastereomers of Ethyl Phosphotriesters of Octanucleotide d(GCCAAACA) by Means of High Performance Complementary (Affinity) Chromatography," Bioorg. Khim., 18(1), 92-99 (1992).
Tashlitskii et al., "Optimization of Conditions for Ion-Pair HPLC of Oligonucleotides," Bioorg. Khim., 2.3(9), 732-741 (1997); Biosis Abstracts, 98, Abstract No. 01070821; only abstract supplied.
Ip et al., "Separation of Nucleosides and Nucleotides by Reverse-Phase High-Performance Liquid Chromatography with Volatile Buffers Allowing Sample Recovery," Analytica Biochemistry, 147(1), 180-185 (May 15, 1985).
Moriyama et al., "New Reversed-Phase High-Performance Liquid Chromatography Column for Oligonucleotide Separation," J. Chromatography, 445(1), 225-233 (Jul. 8, 1988).
Coenen et al., "Optimization of the Separation of the Rp and Sp Diastereomers of Phosphate-Methylated DNA and RNA Dinucleotides," J. Chromatography, 596(1), 59-66 (Apr. 3, 1992).
Fritz et al, "High-Pressure Liquid Chromatography in Polynucleotide Synthesis," Biochemistry, 17(7), 1257-1267 (Apr. 4, 1978).
Kwiatkowski et al., "Use of Reverse Phase Ion Pair Chromatography to Fractionate and Purify DNA Fragments and Monomeric Components of RNA," Acta Chemica Scandinavica B, 38(9), 721-733 (1984).
Zon, "Purification of Synthetic Oligodeoxyribonucleotides," Chapter 14 in High Performance Liquid Chromatography in Biotechnology, Hancock (ed.), John Wiley & Sons, New York, NY, 1990, pp. 301-398.
Green et al, Bio Techniques, 19:5, pp. 836-841, (1995).
Green et al, BioPharm, 10:5 52-62, (May 1997).
Heftman, 5th Edition Journal of Chromatography Library, vol. 51A, pp. A299-A300 (1992).
Rouhi et al, C&EN Washington,(Mar. 16, 1998).
Puresyn, Inc. Communique, (1997).
Gjerde Douglas T.
Haefele Robert M.
Taylor Paul D.
Crane L. Eric
Transgenomic Inc.
Walker William B.
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