Method for growth in tissue culture of normal colonic epithelial

Details Method for growth in tissue culture of normal colonic epithelial Method for growth in tissue culture of normal colonic epithelial

1984-09-26

1988-05-10

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 29, 435 30, 435 34, 435 35, 435244, C12N 500, C12Q 102

Patent

active

047435526

ABSTRACT:
The invention concerns a method for the routine primary culture of human colonic epithelial cells within a monolayer. 12-0-tetradecanoylphorbol-13-acetate (TPA) enhanced DNA synthesis an average of 8 fold when assayed by [.sup.3 h]dThd labeling indices (L.I.) in colonic epithelial cells from familial polyposis patients and in tubular adenomas. No such stimulation by TPA was seen in cells from high-risk patients without familial polyposis, or in cells from low-risk subjects.

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