Plant cell microinjection technique

Details Plant cell microinjection technique Plant cell microinjection technique

1985-08-01

1988-05-10

Warren, Charles F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43524047, 935 53, 935 67, C12N 1500, C12N 502

Patent

active

047435488

ABSTRACT:
Microinjection techniques for plant protoplasts utilize a holding pipette for immobilizing the protoplast while an injection pipette is utilized to inject the macromolecule. In order to manipulate the protoplasts without damage, the protoplasts are cultured for from about 1 to 5 days before the injection is performed to allow for partial regeneration of the cell wall. It was found that injection through the partially regenerated cell wall could still be accomplished and particular compartments of the cell could be targeted. The methods are particularly useful for transformation of plant protoplasts with exogenous genes.

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