Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1996-02-09
1999-03-09
Cintins, Marianne M.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
514 54, A01N 102
Patent
active
058798773
ABSTRACT:
A sperm solution including arabinogalactan is provided which is useful for separating the motile sperm, to produce sperm samples which are suitable for use in a variety of diagnostic and research applications. In one embodiment, the solution includes an effective amount of arabinogalactan to permit washing or separation of the sperm, while maintaining sperm viability during the washing or separation procedure. Using the improved sperm wash, non-sperm substances that are capable of having a detrimental effect on sperm viability, such as seminal plasma, white blood cells, red blood cells, freezing extender agents, sperm debris, and media components, can be removed from the sperm sample. The inclusion of arabinogalactan in the solution has a protective effect on sperm in a sperm sample during processing. In a preferred embodiment, the arabinogalactan is ultrarefined. The improved sperm solution is compatible with sperm samples obtained from variety of different mammals, including human, bovine and equine sperm samples. The washed sperm can be used in a variety of diagnostic or research protocols including infertility testing, sperm toxicology testing and in vitro fertilization.
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Ellington Joanna E.
Oliver Sylvia Adams
Advanced Reproduction Technologies Inc.
Cintins Marianne M.
Jones Dwayne C.
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