Refrigeration – With illuminating or radiant energy means
Patent
1986-12-16
1988-05-10
Bennett, Henry A.
Refrigeration
With illuminating or radiant energy means
34 92, 118 501, 422131, 62514R, F25D 2300
Patent
active
047426900
ABSTRACT:
This invention relates to apparatus for the cryopreparation of biological tissue samples for ultrastructural analysis. The use of the apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a thermally conductive sample holder at a temperature of less than -140.degree. C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymerization of the resin to form an embedded tissue sample.
REFERENCES:
patent: 3679450 (1972-07-01), Beightol
patent: 3745290 (1973-07-01), Harnden, Jr. et al.
patent: 3942519 (1976-03-01), Shock
patent: 4055904 (1977-11-01), Horne
patent: 4056855 (1977-11-01), Kelman
patent: 4063068 (1977-12-01), Johnson et al.
patent: 4100158 (1978-07-01), Hydes et al.
patent: 4120991 (1978-10-01), Ornstein et al.
patent: 4197658 (1980-04-01), Fraser
patent: 4232453 (1980-11-01), Edelmann
patent: 4248821 (1981-02-01), Van Dellen
patent: 4266111 (1981-05-01), Trillwood
patent: 4269713 (1981-05-01), Yamashita et al.
patent: 4278623 (1981-07-01), Niegisch
patent: 4278701 (1981-07-01), von Hagens
patent: 4331591 (1982-05-01), Baylis
patent: 4336691 (1982-06-01), Burstein et al.
patent: 4337240 (1982-06-01), Saklad
patent: 4347671 (1982-09-01), Dias et al.
patent: 4379003 (1983-04-01), Robbins et al.
patent: 4449305 (1984-05-01), Baron et al.
patent: 4510169 (1985-04-01), Linner
patent: 4567847 (1986-02-01), Linner
patent: 4619257 (1986-10-01), Linner et al.
Sleytr, U. B. and Robards, A. W., Understanding the Artefact Problem in Freeze-Fracture Replication: A Review, Journal of Microscopy, 126, 101-122 (1982).
Terracio, L. and Schwabe, K. G., Freezing and Drying of Biological Tissues for Election Microscopy, Journal of Histochemistry and Cytochemistry, 29, 1021-1028 (1981).
G. Smolin and R. Throft, The Cornea, Scientific Foundations and Clinical Practice, 437-464 (1983).
T. Casey and D. Mayer, Corneal Grafting, Principles Practice, 81-86, 271-280 (1984).
A New Technique for Removal of Amorphous Phase Tissue Water Without Ice Crystal Damage: A Preparative Method for Ultrastructural Analysis and Immunoelectron Microscopy, J. of Histochemistry and Cytochemistry; Linner, J., S. Livesey, D. Harrison, A. Steiner; vol. 34, pp. 1123-1135 (1986).
Bennett Henry A.
Board of Regents , The University of Texas System
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