Method for purification of HBs antigen

Details Method for purification of HBs antigen Method for purification of HBs antigen

1978-09-28

1979-07-24

Rose, Shep K.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

Recovery or purification

424 89, A61K 3912

Patent

active

041621921

ABSTRACT:
An improved method for purification of HBs antigen comprising passing a partially purified HBs antigen prepared from blood plasma or serum through a column filled with an anion exchanger which may be equilibrated with a buffer solution having a specific ionic strength and pH level, and further passing the resulting effluent through a column filled with a cation exchanger which may be equilibrated with a buffer solution having a specific ionic strength and pH level. The HBs antigen obtained by the present invention is highly purified and is useful for preparing vaccine for preventing Viral Hepatitis Type B.

REFERENCES:
patent: 3976767 (1976-08-01), Neurath
patent: 4113712 (1978-09-01), Funakoshi

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