Bioassay for chemicals which generate prooxidant states

Details Bioassay for chemicals which generate prooxidant states Bioassay for chemicals which generate prooxidant states

1989-05-08

1993-03-30

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 25, 435 26, 436 35, 436164, C12Q 126, C12Q 132, G01N 2400, G01N 2100

Patent

active

051983362

ABSTRACT:
A bioassay making use of submitochondrial particles to test for the presence of toxic substances which induce prooxidant states in vivo. The assay uses complex I of the electron transport enzymes on the submitochondrial particles which are capable of donating electrons to the toxicant in solution. The presence of any activated oxygen species in the assay solution is detected spectrophotometrically by the adrenochrome reaction.

REFERENCES:
patent: 4808517 (1989-02-01), Blondin et al.
Smith et al., Meth. Enz. 105:505-510 (1984).
Lorentzen et al., Biochemistry 16(7): 1467-1473 (1977).
Abstract and accompanying poster "An in vitro mitochondrial bioassay for predicting acute toxicity in fish", Blondin, Knobeloch, Read and Harkin, Cincinnati, OH, May of 1987.
"Use of Mammalian Mitochondrial Electron Transport Particles for the Detection of Toxic Substances in Fresh Water and Evaluation of Chemical Toxicity", Thesis by Lynda Knobeloch submitted to University of Wisconsin-Madison, Winter of 1988-89.

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