Multilayer analytical element for determination of total cholest

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol

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422 56, 422 57, 436174, 436175, 436177, C12Q 160, G01N 2100, G01N 100

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active

053506758

ABSTRACT:
In a dry type analytical element having at least one water-permeable layer including a porous spreading layer and at least one water-permeable layer, containing an enzyme having cholesterol esterase activity, the improvement which comprises containing a condensate of poly(ethylene oxide) monoalkylphenyl ether and formaldehyde at least in the water-permeable layer containing the enzyme or another water-permeable layer. In the analytical element of the invention, hemolysis does not occur and bound cholesterol is sufficiently decomposed to free cholesterol.

REFERENCES:
patent: 2541991 (1951-02-01), DeGroote
patent: 3925164 (1975-12-01), Beaucamp
patent: 3992158 (1976-11-01), Przybylowicz
patent: 4144129 (1979-03-01), Gruber
patent: 4202938 (1980-05-01), Haeckel
patent: 4212938 (1980-07-01), Gruber
patent: 4258001 (1981-03-01), Pierce
patent: 4275151 (1981-06-01), Esders
patent: 4292272 (1981-09-01), Kitajima

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