Process for the recovery of .alpha.-2-SB-glycoprotein

Details Process for the recovery of .alpha.-2-SB-glycoprotein Process for the recovery of .alpha.-2-SB-glycoprotein

1980-11-20

1982-04-20

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

260112R, 260117, 2601237, 424 85, 424101, 424177, C07G 700

Patent

active

043258670

ABSTRACT:
The present invention provides a process for obtaining .alpha.-2-SB-glycoprotein from physiological solutions thereof by binding to a bindable protein immobilized on an insoluble carrier, wherein the carrier-fixed protein is subsequently washed with a buffer solution of pH 6 to 8.5 and thereafter the .alpha.-2-SB-glycoprotein is eluted with a buffer solution with a pH of 9 or more and isolated from the eluate.

REFERENCES:
patent: 4104266 (1978-08-01), Wickerhauser
patent: 4278594 (1981-07-01), Amrani
Journal of Biological Chemistry, vol. 245, No. 21, 1970, pp. 5728-5736, Mosesson et al.
Journal of Biological Chemistry, vol. 250, No. 16, 1975, pp. 6614-6621, Mosher.
Biochemical J. (1978), 175, 333-336, Vuento et al.
Annals of N.Y. Academy of Sciences, vol. 312 (1978), 178-191, Ruoslahti et al.
Biochem. J. (1978), 169, 55-59, Dessau et al.
Science, vol. 201, Aug. 1978, pp. 622-624, Saba et al.
Nature, vol.-275, 1978, pp. 179-184, Yamada et al.

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