Method for separation of components in a biochemical reaction ut

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic

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436 45, 436 52, 436518, 436523, 436534, 436524, 436806, 436807, 436824, 435971, 435 8, 422681, 422 69, 422 72, 210222, 210223, 210695, G01N 33553, G01N 3500, G01N 3508, G01N 1506, C12Q 166

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active

061501824

ABSTRACT:
A method for combining magnetic and centrifugal extraction techniques in a manner that improves wash efficiency and reduces the relative disadvantages of stand alone magnetic or centrifugal systems is provided. Centrifugation occurs about the rotational axis of a generally circular container with generally vertical sides featuring an inward protruding physical feature designed to retain material that exceeds the density of supernatent liquid and that is bound to magnetic particles during the centrifugation. During centrifugation, an external magnetic field is applied about the rotational axis of the container such that magnetic lines of force penetrate the container in any desired location. The magnetic and centrifugal forces may be independently modulated or modulated in relative unison to suit the hydrodynamic characteristics of a given complex.

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