Method, composition and device for the semiquantitative determin

Details Method, composition and device for the semiquantitative determin Method, composition and device for the semiquantitative determin

1993-10-27

1994-06-14

Housel, James C.

Chemistry: analytical and immunological testing

Metal or metal containing

Mn, te, re, fe, ru, os, co, rh, ir, ni, pd, pt

422 56, 422 57, 436 74, 436 79, 436169, G01N 3320, G01N 3122

Patent

active

053209699

ABSTRACT:
A method, composition and test device for the semiquantitative determination of specific gravity of a test sample are disclosed. The method utilizes a reagent composition capable of producing a detectable and measurable response that correlates to the concentration of cations, and therefore the specific gravity, of the test sample. The reagent composition comprises: a) a polyvalent metal ion having a valence of at least two, like mercuric ion or calcium ion; b) an indicator capable of interacting with the polyvalent metal ion to provide a polyvalent metal ion-indicator complex having a first color; c) a buffer; and d) a suitable carrier. The reagent composition is used in a wet phase specific gravity assay or is incorporated into a carrier matrix, like filter paper, to provide a test pad useful in a dry phase specific gravity assay of a test sample, such as urine.

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