Method of preparing solution enriched in xylanase using low mole

Details Method of preparing solution enriched in xylanase using low mole Method of preparing solution enriched in xylanase using low mole

1992-04-03

1994-06-14

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435201, 435205, 435209, 435814, 435816, C12N 924, C12N 926, C12N 934, C12N 942

Patent

active

053209605

ABSTRACT:
A method for preparing an aqueous solution enriched in xylanase from an aqueous mixture containing cellulase proteins and xylanase is disclosed. The method involves adding an amount of a low molecular weight alcohol to an aqueous mixture containing cellulase proteins and an organic salt so as to precipitate out the cellulase proteins other than EG III from the mixture. The method then involves adding an inorganic salt to the supernate produced in the previous step so as to form a second precipitate and a second supernate and then finally collecting the second precipitate from the second supernate to obtain a precipitate enriched in xylanase.

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