Thermostable alginate lyase from Bacillus steraothermophilus

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase

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4352525, 435832, C12N 988, C12N 120

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active

051399458

ABSTRACT:
A method of producing algal cell wall degrading enzyme by cultivating a thermophilic, bacterial strain of an atypical Bacillus stearothermophilus e.g., W36-7-4 (NRRL B-18394) which produces the alginate lyase in a cultivation medium containing sodium alginate and tryptone, then allowing growth to take place to accumulate alginate lyase, and then recovering the enzyme from the fermentation broth. The novel alginate lyase of this invention is useful as a treatment agent for water cooling systems.

REFERENCES:
Hansen, J. B. et al. "Distribution of Alginate Lyase Activity among Strains of Bacillus circulans", Applied & Environmental Microbiology, vol. 49, No. 4, pp. 1019-1021, 1985.
Wicker-Bockelmann, U. et al., "Alginate Lipase Releases Cell-Bound Lyase from Mucoid Strains of Pseudomonas aeuginosa", Zbl. Bakt. Hyg. A 266, pp. 379-389, 1987.

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