Nucleic acid-free thermostable enzymes and methods of production

Details Nucleic acid-free thermostable enzymes and methods of production Nucleic acid-free thermostable enzymes and methods of production

1997-01-02

1999-01-19

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

530412, 530414, 530422, C12N 912

Patent

active

058612955

ABSTRACT:
The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).

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