Method of analysis or assay for polynucleotides and analyzer or

Details Method of analysis or assay for polynucleotides and analyzer or Method of analysis or assay for polynucleotides and analyzer or

1996-11-27

1999-01-19

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

2041828, 435 9151, 435 911, 435 9152, 435 9153, 435 912, 536 243, 536 2531, 536 2532, C07K 314, C12Q 168, C12P 1934, C07H 2104

Patent

active

058612521

ABSTRACT:
A method of analysis or assay for nucleotides comprises: (1) a step of digesting DNA with a restriction enzyme; (2) a step of discriminating a difference in sequences of the DNA fragments obtained in step (1) above around the 3' termini thereof with a DNA probe and extending the DNA probe by a complementary strand synthesis to fractionate the DNA fragments into groups; and, (3) a step of measuring lengths of the DNA fragments which belong to said groups, or length of the DNA probe extended by said complementary strand extension reaction; wherein the thus measured lengths obtained for every sequence of the bases of the DNA fragments around the 3' termini thereof are employed as fingerprints.

REFERENCES:
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patent: 5376526 (1994-12-01), Brown et al.
patent: 5650274 (1997-07-01), Kambara et al.
Straus et al. Genomic subtraction for cloning DNA corresponding to deletion mutations, Proc. Natl. Acad. Sci. USA. vol. 87, pp. 1889-1893, 1990.
Genomics, 23, 1994, Murakawa et al, pp. 379-389.
DNA Sequence--J. DNA Sequencing and Mapping, vol., 2, 1991, Okubo et al, pp. 137-144.
Nature, vol. 357, Jun. 11, 1992, Mitsuhashi et al, pp. 519-520.

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