Method for removing N-terminal dipeptides from precursor polypep

Details Method for removing N-terminal dipeptides from precursor polypep Method for removing N-terminal dipeptides from precursor polypep

1995-05-19

1996-10-15

Rollins, John W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435212, 435223, C12P 2106, C12N 948, C12N 958

Patent

active

055653308

ABSTRACT:
A method for removing dipeptides from the amino terminus of precursor polypeptides to produce a polypeptide product is presented which comprises contacting the precursor polypeptide for sufficient time to remove the dipeptide with a dipeptidylaminopeptidase (dDAP) from the slime mold, Dictyostelium descoideum, which has a mass of about 225 kilodaltons and a pH optimum of about 3.5. The precursor polypeptides may be made recombinantly and may be analogs of naturally occurring polypeptides.

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