Method for determining histamine by measuring dissolved oxygen

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 27, 435 28, 435183, 435191, 435192, 435225, 4352875, 435814, 435817, 435917, 4352871, 426 52, 426 56, C12Q 126, C12N 900, A23L 131

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055653294

ABSTRACT:
The present invention relates to a method of determining histamine content as a freshness index of food. An examination liquid is injected in a reaction cell, an amount of dissolved oxygen (DO) is recorded through an oxygen sensor and an amplifier in the recorder. Then, an enzymatic reagent having histamine oxidase activity is injected in the reaction cell, a decrease in the dissolved oxygen is recorded in the recorder, and the histamine concentration is determined on the basis of the decrease by a micro computer.

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